In vitro Evaluation of Microleakage in Contaminated Fissures Sealed with GC Fuji Triage Glass Ionomer Cement

Abstract. The aim of this in vitro study was to evaluate the microleakage of Fuji Triage glass ionomer in<br>contaminated pits and fissures, and to compare it with the microleakage of resin-based pit and fissure sealant.<br>Sixty-four extracted human premolar teeth were randomly assigned into two main groups according to the fissure<br>sealant used (Fuji Triage or ClinproTM). Each group was divided into four subgroups according to the different<br>surface conditions as follows: (1= dry condition; 2= water contamination; 3= saliva contamination; 4= saliva<br>contamination and air-drying). The teeth were subjected to thermal cycling (500 cycles at 5oC-55oC) with a dwell<br>time of 30 seconds. The teeth were then immersed in 1% methylene blue for 24 hours, embedded in acrylic resin<br>and sectioned longitudinally in a buccolingual direction. The sections were analyzed for microleakage using a<br>stereomicroscope. Microleakage was evaluated on a three-point rating scale and was analyzed using t and<br>ANOVA tests. Both materials, under different conditions, exhibited dye penetration to some extent. However, Fuji<br>Triage cement showed less microleakage in wet conditions compared to ClinproTM group. Whenever wet (water or<br>saliva) contamination is apparent, the use of Fuji Triage cement as a sealing material would be beneficial to<br>decrease microleakage.